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The HSU Lab

Graduate Institute of Microbiology and Public Health, National Chung-Hsing University, Taiwan

2024

Jiang. M.C., W.L Hsu , C.Y. Tseng, N.S. Lin, Y.H. Hsu*, and C.C. Hu. 2024. Development of a tag-free plant-made interferon gamma production system with improved therapeutic efficacy against viruses. Front. Bioeng. Biotechnol., 11 January. 11:1341340
Chen, L.H., Y.W. Chen, F. Lee, W.L. Hsu , and S.C. Ou*. 2024. Identification and genomic characterization of feline calicivirus from a leopard cat (Prionailurus bengalensis) in Taiwan. The Journal of Veterinary Medical Science., DOI: 10.1292/jvms.23-0522
Fan YC, Chen JM, Chen YY, Hsu WL , Chang GJ, Chiou SS. 2024. Low temperature culture enhances production of flavivirus virus-like particles in mammalian cells. Appl Microbiol Biotechnol, 108(1):242
Chang,Y.C., H. Shimoda, M.C. Jiang, Y.H. Hsu, K Maeda, Y. Yamada, and W.L. Hsu 2024. Gn protein expressed in plants for diagnosis of severe fever with thrombocytopenia syndrome virus. Applied Microbiology and Biotechnology, (2024) 108:303
Kuo, T.C., W.L. Hsu , V.C. Wu, T.R. Jan, P.S. J. Tsai, Y.J. Lee. 2024. Urinary angiotensin-converting enzyme 2 and its activity in cats with chronic kidney disease. Front Vet Sci. 11:1362379.

2022-2023

Takemae, H., Nunomura, Y., Yokota, T. et al. Novel ollusvirus detected in a solitary wild bee species (Osmia taurus) in Japan. Arch Virol 168, 183 (2023). https://doi.org/10.1007/s00705-023-05805-7
Kuan, Chih-Ying, Shan-Chia Ou, Chao-Chin Chang, Pei-Ling Kao, Ruei-Sheng Tsai, Porjai Rattanapanadda, Tsai-Lu Lin, Ken Maeda, Tsun-Li Cheng, Ya-Jane Lee, and et al. 2023. "Epidemiology of Severe Fever with Thrombocytopenia Syndrome in Dogs and Cats in Taiwan" Viruses 15, no. 12: 2338. https://doi.org/10.3390/v15122338
Sittisak T., T. Guntawang, S. Srivorakul, K. Photichai, K. Boonprasert, S. Khammesri, P. Chuammitri, C. Thitaram, W.L. Hsu, R. Thanawongnuwech, and K. Pringproa. 2023. Response of elephant peripheral blood mononuclear cells when stimulated with elephant endotheliotropic herpesvirus glycoprotein B (EEHV-gB). Veterinary Immunology and Immunopathology 258:110577.
Kuan C.Y., T.L. Lin, S.C. Ou, S.T. Chuang, J. P.W. Chan, K. Maeda, T. Mizutani, M.P. Wu, F. Lee, F.T. Chan, C.C. Chang, R.L. Liang, S.F. Yang, T.C. Liu, W.C. Tu, H.Y. Tzeng, C.J. Lee, C.F. Lin, H.H. Lee, J.H. Wu, H.C. Lo, K.C. Tseng, W.L. Hsu*, and C.C. Chou*. 2023. The First Nationwide Surveillance of Severe Fever with Thrombocytopenia Syndrome in Ruminants and Wildlife in Taiwan. Viruses 15(2): 441.
Tu, E.C., W.L. Hsu* and J. T. C. Tzen*. 2023.Strictinin, a Major Ingredient in Yunnan Kucha Tea Possessing Inhibitory Activity on the Infection of Mouse Hepatitis Virus to Mouse L Cells. Molecules. 28(3): 1080.
Liu, YC., G.R. Liao, A. Y. Tsai, C.Y. Tseng, C.Y. Kuan, R.S. Tsai, R. A. Albrecht, R.L. Kuo, I.C. Cheng, W.T. Liang, S.C. Ou*, W.L.Hsu*.2023. NS2 is a key determinant of compatibility in reassortant avian influenza virus with heterologous H7N9-derived NS segment. Virus Research. 324: 199028
Yamada Y, Chuang ST, Tseng CY, Liao GR, Liu SW, Tseng YY, Lin FY, Hsu WL*.2023 Deletion of gene OV132 attenuates Orf virus more effectively than gene OV112. Appl Microbiol Biotechnol. 107(2-3):835-851.
Wang, C.H., C.Y. Tseng, W.L. Hsu*, J.T.C*. Tzen. Establishment of a Cell Line Stably Expressing the Growth Hormone Secretagogue Receptor to Identify Crocin As a Ghrelin Agonist. Biomolecules 2022, 12, 1813.
Li, C.Z.#, H.M. Chang#, W.L. Hsu#, P. Venkatesan, H.C. Lin, and P.S. Lai. 2022. Curcumin-Loaded Oil-Free Self-Assembled Micelles Inhibit the Influenza A Virus Activity and the Solidification of Curcumin-Loaded Micelles for Pharmaceutical Applications. Pharmaceutics 14: 2422. (# Equal contribution.) (IF: 6.321)
Guntawang T., T. Sittisak, S. Srivorakul, K. Photichai, P. Aiumurai, C. Thitaram, N. Sthitmatee, W.L. Hsu, and N. Sookrung and K. Pringproa *. 2023. Development of an immunochromatographic strip test for antigen detection of elephant endotheliotropic herpesvirus in Asian elephants (Elephas maximus). Journal of Virological Methods. 311 (2023) 114627.
Chung, Y.T., C.Y. Kuan, G.R. Liao, R. Albrecht, Y.Y. Tseng, Y.C. Hsu, S.C. Ou, W.L. Hsu*. 2022. A variant NS1 protein from H5N2 avian influenza virus suppresses PKR activation and promotes replication and virulence in mammals. Emerg Microbes Infect. 11(1):2291-2303. (IF: 19.56)
Lin, H.W., M.L Wang, P.J. Tsai, Y.J. Lee, M.C. Hsieh, D.Y. Lu, W.L. Hsu, M.S. Jan, Y.Y. Chang. 2022.The Establishment of a Noninvasive Bioluminescence-Specific Viral Encephalitis Model by Pseudorabies Virus-Infected NF-kBp-Luciferase Mice. Veterinary Sciences. 9 (3): 113.
Guntawang T., T. Sittisak, P. Tankaew, C. Thitaram, V. Langkapin, T. Angkawanish, T. Singhla, N. Sthitmatee, W.L. Hsu, R. Thanawongnuwech, K. Pringproa *. 2022. Development of nonstructural protein-based indirect ELISA to identify active elephant endotheliotropic herpesvirus (EEHV) infection in Asian elephants (Elephas maximus). Animals. 12:1747.
Li, T.H. #, W.L. Hsu#, C.Y. Chen#, Y.C. Chen, Y.C. Wang, M.A. Tsai, I.C. Chen, and C.C. Chang*. 2022. Preparation of Recombinant Glycoprotein B (gB) of Chelonid Herpesvirus 5 (ChHV5) for Antibody Production and Its Application for Infection Detection in Sea Turtles. Scientific Reports. 12:11022. (IF: 4.38)
W.J. Wang, M.C. Chou, Y.J. Lee, W.L. HSU*, and G.J. Wang*. 2022. A simple electrochemical immunosensor based on a gold nanoparticle monolayer electrode for neutrophil gelatinase-associated lipocalin detection. Talanta. 246: 123530 (4 May 2022) (IF: 6.057)

2020-2021

Chen, Y.C., W.Y. Chin, C.C. Chang, S.T. Chuang, and W.L. Hsu*. 2021. Potential Risk Factors Associated with Infection with Bovine Leukaemia Virus in Dairy and Beef Cattle in Taiwan. Pathogens 10(12): 1553 (IF: 3.406)
Liao, G.R., Y.Y. Tseng, C.Y. Tseng, C.Y. Lo, W.L. Hsu*. 2021. The orf virus (ORFV) protein OV20.0 interacts with themicroprocessor complex subunit DGCR8 to regulatemiRNA biogenesis and ORFV infection. FEBS Letters. 595 (2021) 2897-2908. (IF: 4.124)
Tseng,Y.Y., C.Y. Kuan, M. Mibayashi, C.J.Chen, P. Palese, R. A. Albrecht, W.L. Hsu*. 2021. Interaction between NS1 and cellular MAVS contributes to NS1 mitochondria targeting. Viruses.13: 1909. (IF: 5.079)
M.H. Liao, X.R. Wang, W.L. Hsu*, Jason T.C. Tzen*. 2021. Pu’er tea rich in strictinin and catechins prevents biofilm formation of two cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus. Journal of Dental Sciences. Accepted in May 25. 2021.
Lin, H.W., Y.J. Lee, D.J. Yang, M.C. Hsieh, C.C. Chen, W.L. Hsu, Y.Y. Chang*, C.W. Liu*. 2021. Anti-inflammatory effects of Flos Lonicerae Japonicae Water Extract are regulated by the STAT/NF-κB pathway and HO-1 expression in Virus-infected RAW264.7 cells. Int J Med Sci. 2021 Mar 30;18(11):2285-2293
Liao, G.R., Y.Y. Tseng, C.Y. Tseng, Y.P. Huang, C.H. Tsai, H.P. Liu, W.L. Hsu*. 2021. K160 in the RNA-binding domain of the orf virus virulence factor OV20.0 is critical for its functions in counteracting host antiviral defense. FEBS Letters. 595: 1721–1733.
Tseng, Y.Y., G.R. Liao, A. Lien, and W.L. Hsu*. 2021. Current concepts in the development of therapeutics against human and animal coronavirus diseases by targeting NP. Computational and Structural Biotechnology Journal 19 (2021) 1072-1080.
Jiang, M.C., C.C. Hu, W.L. Hsu, T.L. Hsu, N.S. Lin and Y.H. Hsu*. 2020. Fusion of a Novel Native Signal Peptide Enhanced the Secretion and Solubility of Bioactive Human Interferon Gamma Glycoproteins in Nicotiana benthamiana Using the Bamboo Mosaic Virus-Based Expression System. Front. Plant Sci. Nov, 11:594758
Lin, P.R., P.C. Kuo, Y.C. Lia, C.F. Jhuo, W.L. Hsu*, Jason T.C.Tzen*. 2020. Theacrine and strictinin, two major ingredients for the anti-influenza activity of Yunnan Kucha tea. Journal of Ethnopharmacology November, 262: 113190.
Guntawang, T., T. Sittisak, S. Srivorakul1, V. Kochagul, K. Photichai, C. Thitaram, N. Sthitmatee, W.L. Hsu & K. Pringproa. 2020. In vivo characterization of target cells for acute elephant endotheliotropic herpesvirus (EEHV) infection in Asian elephants (Elephas maximus). Scientific Reports. July 10:11402
Jing, H. #, W.L. Hsu#,V. Wu, H.J. Tsai, S.F. Tsai, P. S Tsai, T. S. Lai, and Y.J. Lee*. 2020. Urine hemojuvelin in cats with naturally occurring kidney diseases. Journal of Veterinary Internal Medicine. May 34(3):1222-1230 (#co-first author).
Chen, Y.C., C.C. Chang, W.L. Hsu*, and S.T. Chuang*.2020. Dairy cattle with bovine leukaemia virus RNA show significantly increased leukocyte counts. Vet J. 257:105449 .
Wang W.C., C.Y. Kuan, Y.J. Tseng, C.H. Chang, Y.C. Liu, Y.C. Chang, Y.C. Hsu, M.K. Hsieh, S.C. OU*, W.L. Hsu*. The Impacts of Reassortant Avian Influenza H5N2 Virus NS1 Proteins on Viral Compatibility and Regulation of Immune Responses. 2020. Frontiers in Microbiology. Mar.12; 11:280.
Lin, T.L#, S.C. Ou#, K. Maeda, H. Shimoda, Jacky P.W. Chan, W.C. Tu, W.L. Hsu* and C.C. Chou*. 2020. The First Discovery of Severe Fever with Thrombocytopenia Syndrome Virus in Taiwan. Emerging Microbes and infections. 9: 148-151.
Chen, YC., J.W. Liao, S.C. Chang, and W.L. Hsu*. 2020. Expression frequency of c-kit isoforms and its prognostic potential in canine mammary Tumours. Veterinary and Comparative Oncology. Sep;18:303-314.

2018-2019

Hsieh#, J.C. C.Y. Li#, W.L. Hsu*, S.T. Chuang*. 2019 Dec. Molecular epidemiological and serological studies of Bovine Leukemia Virus in Taiwan. Frontiers in Vet Sci.6: 427. (IF 2.029, 12.8%)
Yamada Y., G.R. Liao1, C.Y. Tseng., Y.Y. Tseng, W.L. Hsu*. 2019 Establishment and characterization of transformed Goat Primary cells by expression of Simian Virus 40 Large T Antigen for orf virus propagations. PLOS One Dec 5;14(12):e0226105 (IF 2.776, 34.8%)
Wu, P.H#., W.L. Hsu#, P.S. Jason Tsai, V.C. Wu, H.J. Tsai, Y.J. Lee. 2019 Identification of urine neutrophil gelatinase-associated lipocalin molecular forms and their association with different urinary diseases in cats. BMC Vet Res. 15: 306 (#co-first author) (IF: 1.792, 22.7%)
Chen, YC., S.C. Chang, Y.H. Huang, Y.J. Lee, C.C. Chang, J.W. Liao, and W.L. Hsu*. 2019 Expression and the molecular forms of neutrophil gelatinase-associated lipocalin and matrix metalloproteinase 9 in canine mammary tumors. Veterinary and Comparative Oncology. 17(3):427-438. (IF: 2.379, 5.6%)
Liao, G.R., Y.Y. Tseng, C.Y. Tseng, F.Y. Lin, Y. Yamada, H.P. Liu, C.Y. Kuan, W.L. Hsu*.2019. Adenosine Deaminase Acting on RNA 1 Associates with Orf Virus OV20.0 and Enhances Viral Replication J. Virology. 93:e01912-18. (IF 4.324, 22.2%)
Richart, S. M.#, Y.L. Li#, Y. Mizushina, Y.Y. Chang, T.Y. Chung, G.H. Chen, J. T.C. Tzen, K.S. Shia and W.L. Hsu*. 2018. Synergic effect of curcumin and its structural analogue (Monoacetylcurcumin) on anti-influenza virus infection. JFDA. 26:1015-1023
Dony Mathew, Wei-Li Hsu*. 2018 Antiviral Potential of Curcumin. Journal of Functional Foods 40: 692-699

2016-2017

Tsai, CF, H.Y. Lin, WL Hsu* and CH Tsai*. 2017. The novel mitochondria localization of influenza A virus NS1 visualized by FlAsH labelling. FEBS OpenBio. 7(12): 1960-1971
Chen JM, Fan YC, Lin JW, Chen YY, Hsu WL, Chiou SS. 2017 Bovine Lactoferrin Inhibits Dengue Virus Infectivity by Interacting with Heparan Sulfate, Low-Density Lipoprotein Receptor, and DC-SIGN. Int J Mol Sci. 18(9). pii: E1957
Li, TH., WL Hsu, YC Lan, G.H. Balazs, T. M. Work, C.T. Tseng, C.C. Chang*.2017. Identification of Chelonid herpesvirus 5 (ChHV5) in endangered green turtles (Chelonia mydas) with fibropapillomatosis in Asia. Bulletin of Marine Science 93(4) 1011-1022
Wang, I.C#., W.L. Hsu#, P.H. Wu, H.Y. Yin, H.J. Tsai, Y.J. Lee. 2017 Neutrophil Gelatinase-Associated Lipocalin in Cats with Naturally Occurring Chronic Kidney Disease. J Vet Intern Med. 31(1):102-108 (# co-first author)
Chen, Y.C., J.W. Liao, W.L. Hsu*, S.C. Chang*. 2016. Identification of the two KIT isoforms and their expression status in canine hemangiosarcomas. BMC Veterinary Research. 12:142.
Lee, M.L. W.L. Hsu, C.Y. Wang, H.Y. Chen, F.Y. Lin, M.H. Chang, H.Y. Chang, M.L. Wong,
K.W. Chan*. 2016. Goatpoxvirus ATPase activity is increased by dsDNA and decreased by zinc ion. Virus Genes. 52(5):625-32.
Liu, Y.F., P.Y. Tsai, S. Chulakasian, F.Y. Lin, and WL Hsu*. 2016. The pseudorabies virus vhs protein cleaves RNA containing an IRES sequence. FEBS Journal. Mar;283(5):899-911

2014-2015

Liu, Y.F., P.Y. Tsai, F.Y. Lin, K.H. Lin, T.J. Chang, H.W. Lin, S. Chulakasian, and WL Hsu*. 2015. Roles of nucleic acid substrates and cofactors in the vhs protein activity of pseudorabies virus. Veterinary Research 46(1):141.
Tseng, YY., GR Liao, GC. Sen, FY Lin, and WL Hsu*. 2015. Regulation of PACT-mediated PKR activation by the OV20.0 protein of orf virus. J. Virology. 89(22):11619-29.
Lin, F.Y., Y.Y. Tseng, K.W. Chan, S.T Kuo, C.H. Yang, C.Y. Wang, M. Takasu, W.L. Hsu* and M.L. Wong* 2015. Suppression of influenza virus infection by the orf virus isolated in Taiwan. Journal of Veterinary Medical Science. 77(9): 1055–1062.
Tseng, Y.Y., F.Y. Lin, S.F. Cheng, D. Tscharke, S. Chulakasian, C.C. Chou, Y.F. Liu, W.S. Chang, M.L. Wong, and W.L. Hsu*. 2015. Functional analysis of the short isoform of orf virus protein OV20.0. J. Virology 89 (9): 4966-4979
Cheng FP, Hsieh MJ, Chou CC, Hsu WL, Lee YJ. 2015. Detection of indoxyl sulfate levels in dogs and cats suffering from naturally occurring kidney diseases. The Veterinary Journal. 205: 399–403
Chen GH, Lin YL, Hsu WL, Hsieh SK, Tzen JTC. 2015. Significant elevation of antiviral activity of strictinin from Pu'er tea after thermal degradation to ellagic acid and gallic acid.
J Food Drug Anal. 2015 Mar;23(1):116-123
Hsu, WL, Chiou HC, Tung KC, Belot G, Virilli A, Wong ML, Lin FY, Lee YJ. 2014. The different molecular forms of urine neutrophil gelatinase- associated lipocalin present in dogs with urinary diseases. BMC Vet Res. 10(1):202. (IF 1.743, 17.829%)
Yang CH, Tan DH, Hsu WL, Jong TT, Wen CL, Hsu SL, Chang PC. Anti-influenza virus activity of the ethanolic extract from Peperomia sui. 2014. J Ethnopharmacol. 155(1):320-5 (IF: 2.939, 4.545% Integrative & Complementary Medicine)
Chiu HC, Richart S, Lin FY, Hsu WL*, Liu HJ*. 2014 The interplay of reovirus with autophagy. Biomed Res Int. 2014:483657. (a review article) (IF: 2.706, 53/165 32.12%, Biotech & Appl Micro)
Hsu, Wei-Li, Chung-Lun Chen, Shi-Wei Huang, Chia-Chen Wu, I-Hsuan Chen, Muthukumar Nadar, Ying-Pong Su, Ching-Hsiu Tsai*.2014 The Untranslated Regions of Classic Swine Fever Virus RNA Trigger Apoptosis. PLoS ONE. 9(2): e88863 (IF=3.535, 14.54% 12/86 in Biology)
Hsu, W.L, Y.S Lin, Y.Y, Hu, M.L Wong, Y.J Lee. 2014 Neutrophil gelatinase-associated lipocalin in dogs with naturally occurring renal diseases. Journal of Veterinary Internal Medicine. Mar-Apr;28(2):437-42 (IF: 2.064, 9.091% in Vet Science)
Chan KW, Lee ML, Yang WC, Wong ML, Hsu WL, Ho CF, Hsieh YC, Wang CY. Differential diagnosis of Goatpox virus in Taiwan by multiplex polymerase chain reaction assay and high-resolution melt analysis. J Vet Diagn Invest. 2014 Mar;26(2):195-202. (IF: 1.232, 30.23% in Vet Science)
Hung YP, Yang YP, Wang HC, Liao JW, Hsu WL, Chang CC, Chang SC. Bovine lactoferrin and piroxicam as an adjunct treatment for lymphocytic-plasmacytic gingivitis stomatitis in cats. Vet J. 2014 Oct;202(1):76-82 (IF: 2.796, 3.448% in Vet Science)
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